In vitro Anti-ageing Activities of the Extracts of Low-Grade Pineapple and Lime Key from Sob Prab Cooperative Limited, Lampang, Thailand

Abstract

In this study, the low‑grade pineapple and lime key from Sob Prab Cooperative Limited, Lampang, Thailand were selected to investigate the anti‑ageing activities. The pineapple (PA‑J) and lime key juices (LM‑J) were squeezed and then the pineapple and lime key residues were extracted by maceration in 95% (v/v) ethanol (PA‑E and LM‑E) and boiled in distilled water (PA‑W and LM‑W). The extraction yields from all extracts ranged from 14.58 to 23.01, with the presence of terpenoids as a phytochemical. For anti-ageing activities of all extracts, it was found that the LM-J extract showed the highest antioxidant activity including free radical scavenging, metal chelation and lipid peroxidation activities, whereas the PA‑E extract exhibited the whitening effect including tyrosinase inhibition activity and anti-melanogenesis on B16F10 cells, and the stimulation of collagen biosynthesis on human dermal fibroblasts. In addition, all extracts did not show cytotoxicity on human dermal fibroblasts at 0.1 mg mL^-1. This study suggested that the PA‑E and the LM extracts might be beneficial to be an active ingredient for anti-ageing cosmetics.